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402 pcdna3 1 zeo expression vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 402 pcdna3 1 zeo expression vector
    402 Pcdna3 1 Zeo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/402+pcdna3+1+zeo+expression+vector/Zeocin/ppr0789041-213-65-69
    Average 99 stars, based on 1 article reviews
    402 pcdna3 1 zeo expression vector - by Bioz Stars, 2026-11
    99/100 stars

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    Produced:

    Article Title: High-resolution kinetics and cellular determinants of antibody response to SARS-CoV-2 over two years after COVID-19 vaccination
    Article Snippet: .. S, S1 and RBD from Wuhan were produced in 398 lentivirus at IDIBAPS, Barcelona; S2 was purchased at Sino Biological; N was expressed in E. coli 399 and His-tag purified at ISGlobal, Barcelona; RBD from Alpha (B.1.1.7) and Delta (B.1.617.2) 400 were both expressed in Expi293 and His-tag purified at CRG, Barcelona; Codon-optimized 401 nucleotide fragments encoding the RBD Omicron subvariants were cloned into a 402 pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific) and recombinant proteins were 403 produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher 404 Scientific) and subsequently purified from supernatant by high-performance chromatography, 405 as previously described63. ..

    Purification:

    Article Title: High-resolution kinetics and cellular determinants of antibody response to SARS-CoV-2 over two years after COVID-19 vaccination
    Article Snippet: .. S, S1 and RBD from Wuhan were produced in 398 lentivirus at IDIBAPS, Barcelona; S2 was purchased at Sino Biological; N was expressed in E. coli 399 and His-tag purified at ISGlobal, Barcelona; RBD from Alpha (B.1.1.7) and Delta (B.1.617.2) 400 were both expressed in Expi293 and His-tag purified at CRG, Barcelona; Codon-optimized 401 nucleotide fragments encoding the RBD Omicron subvariants were cloned into a 402 pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific) and recombinant proteins were 403 produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher 404 Scientific) and subsequently purified from supernatant by high-performance chromatography, 405 as previously described63. ..

    Clone Assay:

    Article Title: High-resolution kinetics and cellular determinants of antibody response to SARS-CoV-2 over two years after COVID-19 vaccination
    Article Snippet: .. S, S1 and RBD from Wuhan were produced in 398 lentivirus at IDIBAPS, Barcelona; S2 was purchased at Sino Biological; N was expressed in E. coli 399 and His-tag purified at ISGlobal, Barcelona; RBD from Alpha (B.1.1.7) and Delta (B.1.617.2) 400 were both expressed in Expi293 and His-tag purified at CRG, Barcelona; Codon-optimized 401 nucleotide fragments encoding the RBD Omicron subvariants were cloned into a 402 pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific) and recombinant proteins were 403 produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher 404 Scientific) and subsequently purified from supernatant by high-performance chromatography, 405 as previously described63. ..

    Expressing:

    Article Title: High-resolution kinetics and cellular determinants of antibody response to SARS-CoV-2 over two years after COVID-19 vaccination
    Article Snippet: .. S, S1 and RBD from Wuhan were produced in 398 lentivirus at IDIBAPS, Barcelona; S2 was purchased at Sino Biological; N was expressed in E. coli 399 and His-tag purified at ISGlobal, Barcelona; RBD from Alpha (B.1.1.7) and Delta (B.1.617.2) 400 were both expressed in Expi293 and His-tag purified at CRG, Barcelona; Codon-optimized 401 nucleotide fragments encoding the RBD Omicron subvariants were cloned into a 402 pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific) and recombinant proteins were 403 produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher 404 Scientific) and subsequently purified from supernatant by high-performance chromatography, 405 as previously described63. ..

    Plasmid Preparation:

    Article Title: High-resolution kinetics and cellular determinants of antibody response to SARS-CoV-2 over two years after COVID-19 vaccination
    Article Snippet: .. S, S1 and RBD from Wuhan were produced in 398 lentivirus at IDIBAPS, Barcelona; S2 was purchased at Sino Biological; N was expressed in E. coli 399 and His-tag purified at ISGlobal, Barcelona; RBD from Alpha (B.1.1.7) and Delta (B.1.617.2) 400 were both expressed in Expi293 and His-tag purified at CRG, Barcelona; Codon-optimized 401 nucleotide fragments encoding the RBD Omicron subvariants were cloned into a 402 pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific) and recombinant proteins were 403 produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher 404 Scientific) and subsequently purified from supernatant by high-performance chromatography, 405 as previously described63. ..

    Recombinant:

    Article Title: High-resolution kinetics and cellular determinants of antibody response to SARS-CoV-2 over two years after COVID-19 vaccination
    Article Snippet: .. S, S1 and RBD from Wuhan were produced in 398 lentivirus at IDIBAPS, Barcelona; S2 was purchased at Sino Biological; N was expressed in E. coli 399 and His-tag purified at ISGlobal, Barcelona; RBD from Alpha (B.1.1.7) and Delta (B.1.617.2) 400 were both expressed in Expi293 and His-tag purified at CRG, Barcelona; Codon-optimized 401 nucleotide fragments encoding the RBD Omicron subvariants were cloned into a 402 pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific) and recombinant proteins were 403 produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher 404 Scientific) and subsequently purified from supernatant by high-performance chromatography, 405 as previously described63. ..

    Transfection:

    Article Title: High-resolution kinetics and cellular determinants of antibody response to SARS-CoV-2 over two years after COVID-19 vaccination
    Article Snippet: .. S, S1 and RBD from Wuhan were produced in 398 lentivirus at IDIBAPS, Barcelona; S2 was purchased at Sino Biological; N was expressed in E. coli 399 and His-tag purified at ISGlobal, Barcelona; RBD from Alpha (B.1.1.7) and Delta (B.1.617.2) 400 were both expressed in Expi293 and His-tag purified at CRG, Barcelona; Codon-optimized 401 nucleotide fragments encoding the RBD Omicron subvariants were cloned into a 402 pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific) and recombinant proteins were 403 produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher 404 Scientific) and subsequently purified from supernatant by high-performance chromatography, 405 as previously described63. ..

    Suspension:

    Article Title: High-resolution kinetics and cellular determinants of antibody response to SARS-CoV-2 over two years after COVID-19 vaccination
    Article Snippet: .. S, S1 and RBD from Wuhan were produced in 398 lentivirus at IDIBAPS, Barcelona; S2 was purchased at Sino Biological; N was expressed in E. coli 399 and His-tag purified at ISGlobal, Barcelona; RBD from Alpha (B.1.1.7) and Delta (B.1.617.2) 400 were both expressed in Expi293 and His-tag purified at CRG, Barcelona; Codon-optimized 401 nucleotide fragments encoding the RBD Omicron subvariants were cloned into a 402 pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific) and recombinant proteins were 403 produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher 404 Scientific) and subsequently purified from supernatant by high-performance chromatography, 405 as previously described63. ..

    Chromatography:

    Article Title: High-resolution kinetics and cellular determinants of antibody response to SARS-CoV-2 over two years after COVID-19 vaccination
    Article Snippet: .. S, S1 and RBD from Wuhan were produced in 398 lentivirus at IDIBAPS, Barcelona; S2 was purchased at Sino Biological; N was expressed in E. coli 399 and His-tag purified at ISGlobal, Barcelona; RBD from Alpha (B.1.1.7) and Delta (B.1.617.2) 400 were both expressed in Expi293 and His-tag purified at CRG, Barcelona; Codon-optimized 401 nucleotide fragments encoding the RBD Omicron subvariants were cloned into a 402 pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific) and recombinant proteins were 403 produced by transient transfection of FreestyleTM 293-F suspension cells (Thermo Fisher 404 Scientific) and subsequently purified from supernatant by high-performance chromatography, 405 as previously described63. ..



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    99
    Thermo Fisher 402 pcdna3 1 zeo expression vector
    402 Pcdna3 1 Zeo Expression Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/402+pcdna3+1+zeo+expression+vector/Zeocin/ppr0789041-213-65-69
    Average 99 stars, based on 1 article reviews
    402 pcdna3 1 zeo expression vector - by Bioz Stars, 2026-11
    99/100 stars
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